The LAMBDA peristaltic pump supplies the liquid mobile phase continuously to the packed chromatography column at a consistent pulsation-free reproducible flow rate.
Isocratic elution requires one buffer and one peristaltic pump.
However, gradient elution is carried out with two buffers and one or two peristaltic pumps:
"Biochemists recommend LAMBDA peristaltic pumps which guarantee pulsation-free application of liquid mobile phase"
Gradient elution with two pumps | Gradient elution with one pump | |
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Two LAMBDA peristaltic pumps are used to mix the eluents for gradient elution.
| Gradient elution can also be carried out with one peristaltic pump: the containers with elution buffer A & B are connected by a siphon or tube at the bottom with a valve to adjust the flow.
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Requirements of pumping system for column chromatography
The most important requirements of the pumping system for column chromatography are:
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Further information about chromatography, fraction collectors and connection of peristaltic pumps could be found at www.lambda-instruments.com/fraction-collector/.